(1) Morphology and staining: Gram-negative diplococci, the cells are kidney-shaped, arranged in pairs, facing each other, the cell diameter is 0.6 to 1.5 μm, and it is located in neutrophils in the cerebrospinal fluid of the patient.
(2) Cultivation characteristics: The nutritional requirements are high and it is a fastidious bacteria. It must grow well on a medium containing serum or a variety of amino acids, inorganic salts and other substances.
(3) Biochemical reaction: Most strains can decompose glucose and maltose, produce acid without gas (negative gonorrhoeae does not decompose maltose, thereby distinguishing it), positive catalase test, positive oxidase test.
(4) Antigens and typing: There are four main types of antigens: capsular polysaccharide group specific antigen, outer membrane protein type specific antigen, lipopolysaccharide antigen and nuclear protein antigen. According to the different group-specific capsular polysaccharide antigens, the bacteria can be divided into A, B, C, D, X, Y, Z, 1916, 29E and W135 by agglutination reaction and agar diffusion test.
At present, the main cause of human illness is group A. Occasionally, 8 groups, C group and 1916 group caused sporadic cases.
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