1. Take back samples such as cervical swabs and swab with 2~3ml saline. After 30 minutes of rest, remove the supernatant, leave a layer of sediment, add equal volume of normal saline, sample, smear, or centrifuge. Clear the operation; 2, before the centrifugation, first remove the centrifuge tube sleeve in the centrifuge, first balance the balance of the tray (guarantee the pointer is in the middle), select the centrifuge tube sleeve of similar weight, put the sample tube, and balance with physiological saline; Place the well-balanced centrifuge tube symmetrically (6 holes in the inner ring, 6 holes in the outer ring, usually put the inner hole first), usually 500 rpm, centrifuge for 3 to 5 minutes, if necessary, precipitate all the cells. It can be centrifuged for 5 minutes at 3000~3500 rpm, 500 rpm in this experiment, and centrifuged for 3 minutes. 4. After centrifugation, the supernatant is removed. A small amount of supernatant can be left. After mixing, directly sample for smear, or aspirate. All the supernatants, then add the same amount of normal saline, mix and sample the smear; 5, sample 3 ~ 5ul, smear with the gun head, painted into a circle of 1cm2 area, labeled (number and information At the same time, the front and back sides can be distinguished to prevent This rubs off,); 6, after the sample is basically dry, the white film can be microscopically examined to see if the sample is clean and the smear is uniform. (If the mucus is still more, the saline can be washed and centrifuged, and the supernatant can be removed. Step) 7. Fix the smear with 95% ethanol for 2 to 3 minutes, and wash with distilled water. (1, wash away excess ethanol; 2, wet the sample, make the dye liquid easy to color) The latest product: rat vascular endothelial cell adhesion molecule 1 (VCAM-1/CD106) ELISA kit human soluble fibrin monomer Complex (SFMC) ELISA kit Human anti-trophoblast cell antibody (ATA) ELISA kit Human CCAAT enhancer binding protein alpha (C/EBPα) ELISA kit Human B-cell lymphoma factor 2 (Bcl-2) ELISA kit Human anti-brain tissue antibody (ABAb) ELISA kit human laminin/laminin (LN) ELISA kit rat composite prostate specific antigen (CPSA) ELISA kit rat low density lipoprotein immune complex (LDL- IC) ELISA Kit Human Concanavalin A (ConA) ELISA Kit Bovine Lymphocyte Factor ELISA Kit Plant Vitamin B6 (VB6) ELISA Kit Human Insulin Autoantibody (IAA) ELISA Kit Human Anti-ganglioside Antibody ( GM1) ELISA kit mouse neonatal thyroxine (NN-T4) ELISA kit
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