Introduction to the steps to properly install the anaerobic incubator

1. Close the sampling indoor and outdoor doors and vacuum check;

2. Connect the gas path and check the gas path. In order to prevent air leakage, if necessary, seal the glue at each joint. Use (1) Anaerobic environment in the operation room: Place the necessary accessories and instruments according to the requirements. And put two non-toxic plastic bags into the operating room; turn on the light through the power supply, turn on the temperature controller, adjust the required temperature and safe temperature;

3. Open the UV sterilization lamp, sterilize indoors, and sterilize according to the specific experiment.

4. The anaerobic incubator should be placed in a place with small temperature difference and convenient operation. Avoid direct sunlight and away from heating equipment, and place it smoothly. 2. Place the gas cylinder and nitrogen bottle in a stable position, and install the pressure reducing valve separately. (including pressure gauge), placed in the appropriate position;

5. The first replacement in the operating room (nitrogen replacement): A. First insert the rubber inlet into the air inlet of the operation chamber and insert the other end into the plastic bag. B. Turn on the nitrogen inlet and open the nitrogen control valve to make two plastics. The bag is full of nitrogen, close the valve 1, and then tighten the bag mouth; C. Put the latex gloves on the flange of the observation plate and tighten it; D. Put the nitrogen in the plastic bag into the operating room until it is completely discharged.

6. Open the deoxidizing agent in the operating room, turn on the deaerator catalytic converter for catalytic deoxygenation, open the Meilan indicator after one hour, observe the discoloration, and do not change the color to the anaerobic environment in the operating room;

7. The third replacement of the operation room (mixed gas replacement): A. Change the air passage to open the air intake valve. When the air is inflated, the sampling chamber is first vacuumed, and the exhaust switch is opened and closed at any time; B. The mixture is full. After the plastic bag, close the air valve, and open the valve, so that the mixture passes through the flow meter, adjust the flow meter, the flow rate is about 10ml per minute; C. gradually mix the mixed gas in the plastic bag into the operation room; D. After the ventilation, the oxygen content of the gas in the operating room is already in a very small amount.

8. The second replacement of the operating room (nitrogen replacement) repeats the nitrogen filling process. The sampling chamber is evacuated first, and the exhaust is opened and closed with the foot switch at any time.

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