[Indoleamine 2,3-dioxygenase elisa assay] mulsin 62596-29-6 standard cystic fibrosis transmembrane transport regulator CFTR antibody. BK channel protein BKchannel antibody. Anamorphic lymphoma kinase ALK antibody Quercetin-7-O-glucoside 491-50-9 standard lycopene 476-28-8 standard activated protein kinase DJunD antibody. genus/threonine protein kinase CKIIα antibody. , 3-Diooxygenase elisa assay] The level of human indoleamine 2,3-dioxygenase (IDO) in the specimen was determined by double antibody sandwich method. The microplate was coated with purified human indoleamine 2,3-dioxygenase (IDO) antibody to prepare a solid phase antibody, and the indoleamine 2,3-dual addition was sequentially added to the microcapsule of the coated monoclonal antibody. Oxygenase (IDO) is then combined with HRP-labeled indoleamine 2,3-dioxygenase (IDO) antibody to form an antibody-antigen-enzyme-labeled antibody complex. After thorough washing, substrate TMB is added for color development. TMB is converted to blue under the catalysis of HRP enzyme and converted to the final yellow color by the action of an acid. The color depth is positively correlated with the indoleamine 2,3-dioxygenase (IDO) in the sample. The absorbance (OD value) was measured at 450 nm using a microplate reader, and the concentration of human indoleamine 2,3-dioxygenase (IDO) in the sample was calculated from a standard curve. [Indoleamine 2,3-dioxygenase elisa experiment]
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