1. Type:
1.1. Cell culture experiment supplies are all sterile, except for glass containers and pasteurpipet, all are plastic sterile products.
1.2. The surface of the TC-grade culture plate is coated with polymer material to allow the cells to adsorb. The culture vessel types include Tflask, plates, dishes, roller bottle, etc., which are used according to the needs of the experiment.
1.3. Plastic sterile pipet: 1 ml, 2 ml, 5ml, 10 ml, 25 ml
1.4. Plastic centrifuge tube: 15 ml, 50 ml, there are 2 different materials, polypropylene (PP) is opaque material, polystyrene (PS) is transparent material, you can choose the appropriate centrifuge tube according to the experimental needs.
1.5. Glass pastuer pipet: 9 inch, used to remove waste culture fluid, etc.
1.6. Glass serum bottle (Pyrex or Duranglassware): 100 ml, 250 ml, 500 ml, 1000ml
2. Cleaning:
2.1. The newly purchased glass serum bottle should be soaked with 0.1 ~ 0.05 NHCl for several hours, and then washed before use.
2.2. The used glass serum bottle is sterilized by high-pressure steam. After washing, rinse it with primary and secondary deionized water respectively, do not add detergent to clean.
3. Sterilization:
3.1. The glass serum bottle for experiment is covered with aluminum foil paper, autoclaved at 121 ° C, 15 lb, 20 minutes, placed in oven and dried.
3.2. Glass pasteur pipet for experiment is sterilized by dry heat at 170 ℃ for 4 hours.
3.3. Liquid or solid waste can be treated with 10% hypochloride solution (hypochlorous acid, ie bleach) or steam autoclaved at 121 ℃, 15 lb, 20 minutes.
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