Free Generation of Monkey Major Histocompatibility Complex (MHC / RhLA) ELISA Kit Beijing Spot
Beijing Jiehui Bio is a professional ELISA kit supplier, ELISA principle, ELISA kit principle, ELISA kit test service, ELISA test, reasonable price elisa quality assurance.
Reagents and equipment needed but not provided
1. 37 ℃ thermostat.
2. Standard specification microplate reader.
3. Precision pipettes and disposable tips
4. Distilled water,
5. Disposable test tubes
6. Absorbent paper
Precautions for monkey major histocompatibility complex (MHC / RhLA) ELISA kit
1. Remove the kit from 2-8 ° C and allow it to equilibrate at room temperature for at least 30 minutes before opening the kit. If the enzyme-coated plates are not used up after opening, the slats should be stored in sealed bags.
2. The sampler should be used at each step of sample addition, and its accuracy should be regularly checked to avoid test errors.
3. It is recommended that all standards and samples be tested in duplicate.
4. Strictly follow the instructions, and the test results must be determined by the microplate reader.
5. To avoid cross-contamination, avoid repeated use of the tip and sealing film in your hand.
6. Other unused reagents should be packed or covered. Do not mix reagents of different batches. Use before warranty.
7. Substrate B is sensitive to light and avoid prolonged exposure to light.
Washing method
Manual plate washing method: throw away the liquid in the enzyme label plate; place several layers of absorbent paper on the experimental table, and force the enzyme plate to pat several times downward; inject at least 0.35ml of the diluted washing solution into the hole and soak it 1- 2 minutes. Repeat this process several times as needed.
Automatic plate washing: If there is an automatic plate washing machine, it should be used in the formal experiment process after being used proficiently
Specimen requirements for monkey major histocompatibility complex (MHC / RhLA) ELISA kit
1. The sample containing NaN3 cannot be detected because NaN3 inhibits the activity of horseradish peroxidase (HRP).
2. The specimens should be extracted as soon as possible after collection. The extraction should be carried out according to relevant literature. The experiment should be carried out as soon as possible after extraction. If the test cannot be performed immediately, the specimen can be stored at -20 ℃, but repeated freezing and thawing should be avoided
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